IN
VITRO SERVICES AND CAPABILITIES |
 |
|
MIR offers in vitro services to augment its in vivo services. In vitro evaluations of discovery compounds can be done with the same cell lines used to perform in vivo assessments of efficacy, tolerance, and drug function. Thus a correlation between in vitro and in vivo measures of drug function can be established. MIR specializes in the testing of anti-cancer agents and has over 200 different murine and human tumor lines to choose from. From this pool of lines, MIR has validated the most frequently requested for in vitro testing ((click here for in vivo models page)). They encompass all major cancer types. Other cell lines can also be validated upon request.
|
 |
|
|
|
Cancer Research Services |
|
|
| |
|
Anti-Proliferation Assays (small scale screening and IC50): Clients can have their compounds tested against the same human tumor cell lines that are used for in vivo pharmacology studies at MIR. The company currently offers IC50 testing in over 20 human cell lines obtained from the ATCC and NCI and are adding more as clients request. Compounds are incubated with cells for 72 hours and viability is measured using MTT.
|
|
|
| |
|
Biomarker Assays: MIR provides biomarker analysis services to clients conducting pharmacology studies by analyzing specific biomarkers in tumor samples by ELISA. Tumors are harvested before and after treatment and extracts prepared. Clients can request ELISA assays to detect biomarker levels specific to their target. |
|
|
| |
|
Flow Cytometry: MIR can culture cells or extract cells from animals (example: immune cells from spleen) for analysis by flow cytometry. The company can subcontract to the University of Michigan for analysis utilizing their state-of-the-art equipment and highly trained staff. |
|
|
| |
|
 |
Cell Adhesion Assays: MIR in vitro Services can treat tumor cell lines with client compounds and then assay for changes in cell surface marker(s) expression in a 96 well plate assay that uses antibodies to mediate cell adhesion.
Don’t see the type of assay you are looking for? Please contact Frank Urban, MIR's Sr. Manager of Business Development. MIR's staff has decades of pharmaceutical research experience and may be able to perform your special request studies. |
|
|
Inflammation Research Services |
|
|
|
Cytokine Release Assays: In support of our Inflammation Division MIR is offering cytokine release assays. Cytokine profiles can be determined from in vitro cell-based or in vivo samples from compound/LPS-treated cells or animals. |
|
|
| |
|
Cell Viability (IC50): Inflammatory cells can be induced with LPS and then treated with compounds. Response to compounds is monitored by cell proliferation (MTT) or cytokine profile changes. |
|
|
|
| |
|
|
Biomarker Assays: Similar to those services offered to oncology clients, MIR offers ELISA assays to monitor inflammation biomarkers in both in vitro and in vivo studies. |
|
|
|
| |
|
|
Cell Adhesion Assays: MIR can monitor cell adhesion molecule expression levels as well as cell-cell interactions in response to treatment with clients’ compounds. |
|
|
|
| |
|
|
More assays are being developed so if you don’t see what you are looking for please contact MIR to determine if we can help! |
|
|
|
| |
|
|
