MIR
has been licensed by the Xenogen Corporation to
perform bioluminescence and fluorescence imaging
for our clients, even if they do not have a license.
MIR utilizes Xenogen's a state-of-the-art IVIS®
imaging system to capture publication quality
bioluminescence or fluorescence images of disease
states and drug induced changes in physiology.
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The
IVIS® Imaging System is physically calibrated
and measures absolute light emitted from an
animal or sample. This allows MIR to make
meaningful comparisons between different mice
or experiments conducted at different times.
This system also has Xenogens XFO-12 Fluorescence
Option which provides the option of fluorescent
imaging in addition to bioluminescent imaging
for both in vivo and in vitro applications.
MIR can readily image fluorescence from eGFP,
FITC, Ds Red, Cell Tracker® orange, Cy5.5,
Alexa fluor® 660 and 680 and indocyanine
Green as well as other similar dyes and fluorescent
proteins.
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Cells
or animals can be genetically altered to express
fluorescent proteins or enzymes capable of cleaving
a luminescent substrate (luciferase). These genes
can be constitutively expressed to indicate tumor
burden or conditionally expressed and used as
a reporter for a specific gene or function of
interest. MIR has developed a number of assay
systems for non-invasive assessment of drug function.
Applications
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Tumor
Diagnosis
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Physiologic
changes
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Apoptosis
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P53
Activation
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Gene
Expression
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Tracking
of Metasasis
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